DEEX-BIO

DEEX-BIO is a device specializing in the electroextraction of bioproducts.

"Electroextraction Project" : From 2009 to 2011, BETA tech was a part of the group of SMEs (Small and Medium-size Enterprises) participating in the European consortium focusing on yeast biotechnology. (http://www.electroextraction.org/)

Electroinduced modifications of the cell envelope lead to the leakage of a portion of intracellular molecules such as ions, pigments or proteins from cells. Electropulsation can thus be used for the effective extraction of cytoplasmic content with a preserved functional activity.

The release of macromolecules is controlled by both osmotic conditions and electric field strength. High yields of intracellular enzymes from yeasts can be obtained by application of a series of high voltage electric pulses in a flow process.

Using this technology, reports show that the specific activities of the enzymes are higher than those obtained by conventional extraction methods.

Electroextraction offers an efficient downstream bioprocessing of biopharmaceutical proteins or various compounds of vegetal origin.

"Electric system containing two ELECTRO cell S20 unipolar generators (with a 24V alimentation), one electrodes stimulator for each generator, the inversor to deliver the bipolar pulsations to the pulsing chamber, and a picoscope connected to an ordinator to follow and visualize the impulsions delivered in real time. The load was the flow of yeasts in the pulsing chamber. The pump is used to distribute the yeasts suspension in water through the pulsing chamber."

The master and slave complete set up.

References:

Teissié J, Eynard N, Vernhes MC, Bénichou A, Ganeva V, Galutzov B, Cabanes PA. Recent biotechnological developments of electropulsation. A prospective review. Bioelectrochemistry. 2002 Jan;55(1-2):107-12.

Ganeva V, Galutzov B, Teissié J. High yield electroextraction of proteins from yeast by a flow process. Anal Biochem. 2003 Apr 1;315(1):77-84.

Ganeva V, Galutzov B, Teissié J. Flow process for electroextraction of intracellular enzymes from the fission yeast, Schizosaccharomyces pombe. Biotechnol Lett. 2004 Jun;26(11):933-7.

http://www.electroextraction.org/