ELECTRO cell B10 HVLV

P ≥ TP + TI + TN + 20 060μs

Ti ≥ 20 000 µs.

P ≥ TP + TI + TN + 20060 µs and TI ≥ 20 000 µs

P ≥ Tp + Ti + Tn + 60µs

Square wave forms with both TP Inv and TN Inv activated

Square wave forms with TN Inv activated

Square wave forms with TP Inv activated

Square wave forms

Various types of electrodes can be connected to ELECTRO cell devices that provide a uniform electric field:

For cell suspensions:

- Two parallel plate electrodes embedded in a standard electroporation cuvette (2, 4 or 8mm apart) connected to the power unit by a cuvette-holder. A connection box makes the generator compatible with most of the commercialized cuvette chambers.

For adherent plated cells, electropulsation may be carried out directly in the well:

- Two stainless-steel parallel plate electrodes with an interelectrode distance of 2, 5, 8 and 10mm.

References:

André FM, Gehl J, Sersa G, Préat V, Hojman P, Eriksen J, Golzio M, Cemazar M, Pavselj N, Rols MP, Miklavcic D, Neumann E, Teissié J, Mir LM. Efficiency of high- and low-voltage pulse combinations for gene electrotransfer in muscle, liver, tumor, and skin. Hum Gene Ther. 2008 Nov;19(11):1261-71.

Hojman P, Gissel H, Andre FM, Cournil-Henrionnet C, Eriksen J, Gehl J, Mir LM. Physiological effects of high- and low-voltage pulse combinations for gene electrotransfer in muscle. Hum Gene Ther. 2008 Nov;19(11):1249-60

Kanduser M, Miklavcic D, Pavlin M. Mechanisms involved in gene electrotransfer using high- and low-voltage pulses--an in vitro study. Bioelectrochemistry. 2009 Feb;74(2):265-71.

Bureau MF, Wasungu L, Jugé L, Scherman D, Rols MP, Mignet N. Investigating relationship between transfection and permeabilization by the electric field and/or the Pluronic® L64 in vitro and in vivo. J Gene Med. 2012 Mar;14(3):204-15.

Orio J, Coustets M, Mauroy C, Teissie J. Electric field orientation for gene delivery using high-voltage and low-voltage pulses. J Membr Biol. 2012 Oct;245(10):661-6

ELECTRO cell B10 HVLV is a bipolar square wave generator specializing in gene delivery.

HVLV mode: An efficient electrotransfer can be obtained by the successive delivery of a high voltage short duration pulse (HV) inducing membrane destabilization, then a low voltage long duration pulse (LV) allowing DNA electrophoresis in the cytosol.

For in vitro and in vivo experiments, ELECTRO cell B10 HVLV offers the possibility to set all relevant parameters, in order to reach the best efficiency for gene delivery.

Transfection parameters:

On the welcome page, choose:

Voltage level (U) between 0-1 000V (by 4V step)

Length pulse (T) between 5µs- 50ms (by 5µs step)

Period of the pulse repetition (P) between 0,1 ms -10 000 ms (by 5 µs step)

Number of pulse repetition (R) up to 10 000 or infinite (by 1 unit step)

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ELECTRO cell B10 HVLV